Recombinant Yellow Fever Virus Envelope Protein, sFc-tagged
Specification
Related Products
| Cat#: |
YEL-625 |
| Product Name: |
Recombinant Yellow Fever Virus Envelope Protein, sFc-tagged |
| Description: |
This Yellow Fever virus Envelope protein has been manufactured in insect cells in response to the need for a highly purified, concentrated protein for use in further vaccine development and serological based diagnostic assays. |
| Gene: |
Envelope |
| Species: |
YFV |
| Source: |
Insect cells |
| Synonyms: |
Yellow Fever Virus Envelope |
| Formulation: |
Presented in Dulbecco’s phosphate buffered saline (DPBS) pH 7.4. |
| Notes: |
This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations. |
| Tags: |
C-terminal sheep Fc |
| Background: |
Translation of the positive-stranded viral genomic RNA in infected cells results in the synthesis of a polyprotein precursor of 3411 amino acids, which when proteolytically processed, generates 10 viral proteins. These are (from the amino- to carboxi-terminus of the precursor polyprotein): C; prM/M; E; NS1; NS2A; NS2B; NS3; NS4A; NS4B and NS5. The first three are viral structural proteins that form the virus particle together with the RNA molecule, namely capsid (C, 12–14 kDa), membrane (M of 8 kDa, its precursor prM of 18–22 kDa), and envelope (E, 52–54 kDa). These are encoded in the first quarter of the genome. The remainder of the genome encodes the non-structural proteins (NS), numbered from 1 to 5 (NS1 to NS5) in accordance with the order of synthesis.
E protein is required for receptor binding and viral attachment, fusion, penetration, hemagglutination, host range and cell tropism. It also has an important role in immunological anti-virus response, eliciting neutralizing antibodies and inducing protective response. Native E protein forms homodimers and it undergoes conformational rearrangement into a fusogenic homotrimer after entering cells by receptor-mediated endocytosis. This conformational change occurs in the lower pH environment of the endosome where viral lipid envelope fusion with endosomal membrane, releases the nucleocapsids into the cells cytoplasm. Each E protein monomer has a molecular mass of 50-55 kDa and has three distinct domains: domain I, II and III. Domain III is the immunoglobulin-like receptor binding domain and is recognized by virus-neutralizing antibodies, making it a target for diagnostic assays.
Yellow fever continues to be a public health concern inmany countries of Africa and the Americas. It is estimated that 200 000 cases and 30 000 deaths are attributable to yellow fever annually. |
For research or industrial raw materials, not for personal medical use!
