Transmembrane proteins (TP) can be divided into ion channel proteins, transport proteins, G protein-coupled receptors (GPCR), and other receptors according to different functions. The development of innovative drugs targeting transmembrane proteins has always been a research hotspot in the medical field. For example, some drugs exert their effects by interacting with transmembrane proteins on the cell membrane, such as receptors or transporters. Since multiple transmembrane proteins with complex structures are difficult to purify and have low expression levels, it is crucial to find relevant expression systems.
Creative BioMart provides unique transmembrane protein expression services, which can promote research in the field of biomedicine, including antibody drug development, structural and functional research, expression and purification, as well as related disease diagnosis and treatment.
Figure 1. Representative examples of therapeutically-relevant membrane protein structures. (Gulezian E, et al., 2021)
Our Transmembrane Protein Expression Platforms
Creative BioMart has specially developed three major transmembrane protein expression platforms for the development of IVD reagents and biomarkers.
Figure 2. Different formats for presenting membrane proteins for affinity selection experiments. (Huang R, et al., 2016)
Virus-like Particles (VLP) Platform
Strong immunogenicity.
Relatively high abundance.
Can be used for immunization, ELISA, SPR, and BLI.
Full-length transmembrane protein in intact native conformation.
With our advanced VLP platform, we can produce complex membrane proteins into soluble, high-concentration proteins that will support the production of antibodies, the screening of drugs, and the immunization of animals for IVD.
Detergent Micelle Platform
Full-length proteins with multiple transmembrane domains can be expressed.
Able to accurately quantify.
Widely used in immune, ELISA, and cell assays.
Creative BioMart has established a complete detergent micelle platform and screened several very effective detergents that can solubilize and purify transmembrane proteins and maintain their native and correct conformation in detergent-containing solutions.
Nanodisc Platform
Natural conformation with complete biological activity.
Suitable for detergent-interfering experiments without detergent contamination.
Can be used directly in phage screening tests.
Widely used for immunization, ELISA, SPR, and BLI.
Creative BioMart offers the Nanodisc platform that efficiently and gently assists in the extraction of membrane proteins while maintaining their conformation. The obtained proteins have higher purity and are more widely used in IVD.
Our Service Process for Transmembrane Protein Expression
Creative BioMart provides corresponding service processes and schedules based on your choice of service platform.
VLP Platform
VLP Platform
Plasmid Construction
About 2 weeks
Small-scale Expression
About 1 week
Large-scale Expression and Purification
About 1 week
QC
< 1 week
Total
> 1 month
Detergent Micelle Platform / Nanodisc Platform
Detergent Micelle Platform / Nanodisc Platform
Plasmid Construction and Preparation
About 2 weeks
Small-scale Expression and Optimization
< 1 week
Target Protein Expression and Purification
About 1 week
QC
< 1 week
Total
About 1 month
Deliver Results
Purified protein with a purity of over 85%.
Standard COA report and data sheet, including label information, molecular weight, electrophoresis parameters, protein expression, concentration, purity, SDS-PAGE, etc.
Reasons to Choose Us
Possess the development, customization, and service capabilities of specific proteins.
Advanced protein expression technology and rich protein expression experience.
Development and preparation of proteins with low solubility, many disulfide bonds, difficult or no expression, and low expression levels.
Competitive price.
High-quality service to achieve in-depth personal customization.
Creative BioMart focuses on IVD proteins, we can provide services such as small-scale and large-scale protein production for IVD and the development of recombinant proteins, antibodies, and bioanalytical tests, aiming to provide global pharmaceutical companies and life science research institutions with high-quality IVD protein service. If you are interested in our services, please contact us and we will provide you with detailed assistance.
References
Gulezian E, Crivello C, Bednenko J, et al. (2021). Membrane protein production and formulation for drug discovery[J]. Trends in Pharmacological Sciences. 42(8): 657-674.
Huang R, Kiss M M, Batonick M, et al. (2016). Generating recombinant antibodies to membrane proteins through phage display[J]. Antibodies. 5(2): 11.
Figure 1. Representative examples of therapeutically-relevant membrane protein structures. (Gulezian E, et al., 2021)
Figure 2. Different formats for presenting membrane proteins for affinity selection experiments. (Huang R, et al., 2016)
