Betula verrucosa is a prevalent allergen that can be inhaled, causing allergic reactions when the human body comes into contact with or ingests specific components of this substance. Current research shows that allergy is primarily related to pollen. The pollen of birch bark is highly allergenic due to its large inflorescences and easy dispersal of pollen. Allergy sufferers may experience allergic reactions such as asthma, allergic rhinitis, and conjunctivitis. Currently, in vitro diagnostics (IVD) for birch allergy usually require a series of allergy tests, including skin prick testing and serum IgE testing.
Main Steps of IVD for Betula verrucosa Allergy
IVD for Betula verrucosa allergy is usually achieved by detecting specific IgE antibodies against Betula verrucosa pollen in the patient's serum.
Western Blotting
This diagnostic method detects IgE antibodies at the protein level. The Betula verrucosa pollen extract was subjected to SDS-PAGE electrophoresis, then transferred to a membrane, reacted with the patient's serum, and finally developed with enzyme-labeled secondary antibodies.
Immunohistochemistry
This method involves fixing Betula verrucosa pollen extract on a glass slide, reacting it with the patient's serum, and then adding fluorescently labeled secondary antibodies for color development. This method can detect the presence of specific IgE antibodies against Betula verrucosa pollen in patient serum and allows for semi-quantitative analysis of allergens in pollen extracts.
Creative BioMart provides high-quality recombinant Betula verrucosa allergen proteins used for IVD, including ELISA, lateral flow assay, western blot, and other immunoassays.
High purity. Produced in cell culture or microorganisms through genetic engineering, this purification process is relatively simple and scalable.
Good stability. Compared with natural allergens, recombinant allergens are more resistant to heat, acid, and alkali, and are less susceptible to denaturation and inactivation.
Good quality and low price. Large-scale production also reduces production costs.
Higher safety and repeatability.
Our Outstanding Advantages
We own a proficient technical team, top-of-the-line scientific research equipment, and cutting-edge technology to provide unparalleled services.
Our diverse range of IVD products caters to the varied needs of customers, and we strive to offer comprehensive scientific research support.
We place utmost importance on service quality, with a commitment to providing timely and precise IVD-related services, ensuring our customers receive an optimal scientific research experience.
In addition, Creative BioMart also offers a series of allergen proteins and protein-related services to provide customers with high-quality, low-cost active recombinant proteins to meet different needs and assist in preclinical drug development.
As an IVD raw material supplier, Creative BioMart offers an array of premium IVD proteins for scientific research and preclinical diagnosis. In addition, our services extend to provide customizable protein solutions that cater to diverse requirements in IVD reagents, drug R&D, and biotechnology research, vaccine production, among other domains. Please contact us for details if you are interested in our products or services.
Reference
Ramirez J, Carpizo J A, Ipsen H, et al. (1997). Quantification in mass units of Bet v 1, the main allergen of Betula verrucosa pollen, by a monoclonal antibody based‐ELISA[J]. Clinical & Experimental Allergy. 27(8): 926-931.
