Platanus acerifolia is one of the major sources of outdoor allergens and is capable of inducing allergic asthma, rhinitis, contact dermatitis, and other allergic diseases in humans. Currently, it is found that Platanus acerifolia allergen extract mainly contains three allergenic proteins, namely Pla a 1, Pla a 2, and Pla a 3. With the development of molecular biology technology, the use of genetic engineering to prepare recombinant allergens for clinical diagnosis, treatment, and research of allergic diseases has become one of the development directions of the allergy discipline. Recombinant allergens can lay the foundation for the subsequent transformation of hypoallergenic allergens and the acquisition of allergen nucleic acid vaccines.
Main Steps of IVD for Platanus acerifolia Allergy
ImmunoCAP
The pollen extract of Platanus acerifolia was used as an antigen to react with the IgE antibody in the patient's serum, and then a fluorescently labeled secondary antibody was added. Finally, a CAP detector was used to detect the intensity of the fluorescence signal to determine whether the patient was allergic to sycamore pollen.
Western blotting
The Platanus acerifolia pollen extract was subjected to SDS-PAGE electrophoresis, then transferred to a membrane for reaction with the patient's serum, and finally developed with enzyme-labeled secondary antibodies. This method can detect the presence of specific IgE antibodies against pollen in the patient's serum.
Creative BioMart provides high-quality recombinant Platanus acerifolia allergen proteins used for IVD, including ELISA, lateral flow assay, western blot, and other immunoassays.
High purity. No contamination by external toxic agents or pathogenic microbes.
High specificity and sensitivity. The molecular structure and biological activity of recombinant allergens are similar to natural allergens and can effectively stimulate immune responses in allergic patients.
Easy to scale up production. Process amplification enables the acquisition of a multitude of specific proteins, without the restriction imposed by the content of natural extracts or materials.
Higher safety and repeatability.
Our Outstanding Advantages
Our company has a skilled technical team, state-of-the-art scientific research equipment, and cutting-edge technology to deliver unparalleled services.
With a diverse range of IVD products, we cater to the varying needs of our clients and offer all-encompassing scientific research support.
We strive to provide timely and accurate IVD-related services, ensuring that our customers receive the best scientific research experience possible.
In addition, Creative BioMart also offers a series of allergen proteins and protein-related services to provide customers with high-quality, low-cost active recombinant proteins to meet different needs and assist in preclinical drug development.
Creative BioMart provides medical research and preclinical diagnostics with a range of efficient and reliable IVD proteins. In areas such as IVD reagents, drug development, biotechnology research, and other related fields, we offer custom protein services to meet specific requirements. For more information about our products and services, please contact us.
Reference
Ni W W, Huang W, Wu D Q, et al. (2017). Expression and purification of a major allergen, Pla a 1, from Platanus acerifolia pollen and the preparation of its monoclonal antibody[J]. Molecular Medicine Reports. 16(3): 2887-2892.
