The expression products of mammalian cells are closest to natural higher biological protein molecules in terms of molecular structure, physical and chemical properties, and biological functions. Proteins processed through complex post-translational processes can be used in a variety of genetic engineering applications. The system is used to produce recombinant antigens, antibodies, enzymes, and other proteins for the development of immunoassay reagents, diagnostic kits, and biosensors to improve the accuracy and sensitivity of in vitro diagnostics (IVD).
Figure 1. Cell line generation and development for cell culture processes for the generation of recombinant proteins of interest. (Wurm F M, 2004)
Creative BioMart uses CHO and HEK293 as expression hosts for culture to produce high-quality recombinant proteins and antibodies for IVD.
Advantages of Our Mammalian Cell Protein Expression System
Able to achieve post-translational modification and correct protein folding and renaturation.
Protein activity is closer to natural protein.
High expression level.
Highly efficient promoters control protein expression.
Suitable for large-scale protein expression.
Creative BioMart assists in the research and development of IVD and produces large quantities of high-purity target proteins to meet customer needs.
Mammalian Cell Protein Expression Service Items
Creative BioMart provides customers with services ranging from codon optimization, plasmid synthesis, protein purification, as well as property analysis to meet different needs.
Service Item
Service Process
Time (days)
Deliverables
Expression Vector Construction
Codon optimization and gene synthesis
15-20
An expression vector containing the target gene
Sequencing report
Recombinant plasmid
PCR amplification connection
Transform into E. coli strains
Obtain the correct recombinant plasmid
Small-scale Expression
Preparation of transfection-grade recombinant plasmids
9-11
Related identification results
Transiently transfect HEK293, CHO, and other cells
Detection of expression products
Large-scale Expression and Purification
Expand cultured cells and transfect
8-9
Purified protein
Related identification results
Protein purification (chromatography)
Additional Services
Label removal services
2-5
Optimized high-quality protein
Sterilization by filtration
Endotoxin removal
Freeze-drying
QC
Purity and concentration test
3-5
QC report
Provide QC report
Reasons to Choose Us
Rich experience in protein expression, including various types of proteins.
High success rate, multiple proprietary experimental technologies
One-stop service from codon optimization to protein expression and purification.
Suitable for large-scale recombinant antibody production.
The cycle is short and the target protein can be delivered quickly.
Personalized customized services to meet the needs of different customers.
Creative BioMart focuses on IVD proteins, we can provide services such as small-scale and large-scale protein production for IVD and the development of recombinant proteins, antibodies, and bioanalytical tests, aiming to provide global pharmaceutical companies and life science research institutions with high-quality IVD protein service. If you are interested in our services, please contact us and we will provide you with detailed assistance.
Reference
Wurm F M. (2004). Production of recombinant protein therapeutics in cultivated mammalian cells[J]. Nature biotechnology. 22(11): 1393-1398.
Figure 1. Cell line generation and development for cell culture processes for the generation of recombinant proteins of interest. (Wurm F M, 2004)
