The cell-free protein expression system is a kind of exogenous DNA or mRNA as a template, using protein synthesis machinery, protein folding factors, and other related enzymes in cell extracts, by adding amino acids, T7 polymerase, and energy substances. Suitable for expression of transmembrane and toxic proteins. The production of recombinant proteins for immunoassays is one of the most important applications of cell-free expression systems for in vitro diagnostics (IVD). These proteins can be used as antigens or antibodies to detect disease markers, such as viruses, bacteria, or fungi. Additionally, the system could be used to produce protein drugs for therapeutic purposes, which can be administered directly to patients to treat or alleviate symptoms of disease. Creative BioMart supports IVD and produces large quantities of high-purity target proteins to meet customer needs.
Figure 1. General scheme depicting the overall process of cell-free protein production. (Dondapati S K, et al., 2020)
Advantages of Our Cell-free Protein Expression System
A large amount of toxic protein expression can be performed without the restriction of cell physiology.
Increase activity and solubility of the product.
The product is not degraded by intracellular proteases.
The reaction can be carried out in a short time.
No complicated downstream processing is required.
Suitable for high-throughput expression.
It can improve the expression effect of the target protein.
Creative BioMart assists in the research and development of IVD and synthesizes large quantities of high-purity target proteins to meet customer needs.
Cell-free Protein Expression Service Items
Creative BioMart provides customers with services ranging from codon optimization, plasmid synthesis, small-scale amplification, protein purification, as well as property analysis to meet different needs.
Service Item
Service Process
Time (days)
Deliverables
Plasmid Construction
Codon optimization and gene synthesis
15-20
Recombinant plasmid
Sequencing report
PCR amplification
Ligate to pET vector
Obtain the correct recombinant plasmid
Small-scale Protein Expression and Optimization
Multi-condition optimization
7-13
Related identification results
SDS-PAGE electrophoresis verification
Determine optimal reaction condition
Expression and Purification
1-10mL large-scale expression
Purified protein
Related identification results
Identify and optimize purification conditions (chromatography)
Additional Services
Label removal services
2-5
Optimized high-quality protein
Sterilization by filtration
Endotoxin removal
Freeze-drying
QC
Purity and concentration test
3-5
QC report
Provide QC report
Reasons to Choose Us
One-stop service from gene synthesis to protein expression.
With a complete platform, customized and guaranteed services can flexibly meet your different needs.
With a high-throughput screening platform, the optimal expression conditions can be quickly and efficiently obtained.
The cycle is fast, and the purified protein can be delivered in a short time.
Meet the needs of large-scale industrialization.
Provide endotoxin detection and removal services.
Creative BioMart focuses on IVD proteins, we can provide services such as small-scale and large-scale protein production for IVD and the development of recombinant proteins, antibodies, and bioanalytical tests, aiming to provide global pharmaceutical companies and life science research institutions with high-quality IVD protein service. If you are interested in our services, please contact us and we will provide you with detailed assistance.
Reference
Dondapati S K, Stech M, Zemella A, et al. (2020). Cell-free protein synthesis: A promising option for future drug development[J]. BioDrugs. 34(3): 327-348.
Figure 1. General scheme depicting the overall process of cell-free protein production. (Dondapati S K, et al., 2020)
