Yeast is a common host for protein production from recombinant DNA, used in the field of genetic engineering due to its relatively easy genetic manipulation, fast growth, and high expression. As an exogenous protein expression system, the yeast expression system has the advantages of both prokaryotic and eukaryotic expression systems and can modify the expressed protein by processes such as glycosylation to ensure the natural conformation of the protein. In the field of in vitro diagnostics (IVD), yeast protein expression service can produce recombinant proteins used in diagnostic reagents, kits, or biosensors for disease screening, diagnosis, and treatment monitoring.
Figure 1. Main steps of protein production in yeasts.
Advantages of Our Yeast Protein Expression System
Convenient and simple operation.
The price and cultivation cost are low, and the product is easy to separate.
Genetic stability.
Post-translational modification processing functions such as glycosylation, fatty acylation, and protein phosphorylation.
The exogenous protein has high expression and good activity.
Strong promoters, including AOX1, GAP, FLD1, etc.
Creative BioMart assists in the research and development of IVD and synthesizes large quantities of high-purity target proteins to meet customer needs.
Yeast Protein Expression Service Items
Creative BioMart provides customers with services ranging from codon optimization, plasmid synthesis, small-scale amplification, protein purification, as well as property analysis to meet different needs.
Service Item
Service Process
Time (days)
Deliverables
Expression Plasmid Construction
Codon optimization and gene synthesis
15-20
An expression vector containing the target gene
Sequencing report
E. coli containing the expression plasmid
Plasmid instructions
PCR amplification connection
Transform into E. coli strains
Obtain the correct recombinant plasmid
Transformation and Strain Identification
Large-scale amplification of recombinant plasmids and linearized electrotransformation of hosts such as Pichia pastoris
10-13
Related identification results
PCR analysis validation
Screening of high-copy strains through antibiotics
Large-scale Protein Expression and Purification
Select a small amount of transformed positive strains for small-scale expression, as well as evaluate and optimize protein expression
7-12 / 15-25
Purified protein
Related identification results
Identify strains and optimize expression conditions
Protein purification (chromatography)
Additional Services
Label removal services
2-5
Optimized high-quality protein
Sterilization by filtration
Endotoxin removal
Freeze-drying
QC
Purity and concentration test
3-5
QC report
Provide QC report
Reasons to Choose Us
One-stop service from gene synthesis to protein expression.
With a complete platform, customized and guaranteed services can flexibly meet your different needs.
With a high-throughput screening platform, the optimal expression conditions can be quickly and efficiently obtained.
The cycle is fast, and the purified protein can be delivered in a short time.
Meet the needs of large-scale industrialization.
Provide endotoxin detection and removal services.
Creative BioMart focuses on IVD proteins, we can provide services such as small-scale and large-scale protein production for IVD and the development of recombinant proteins, antibodies, and bioanalytical tests, aiming to provide global pharmaceutical companies and life science research institutions with high-quality IVD protein service. If you are interested in our services, please contact us and we will provide you with detailed assistance.
Reference
Vieira Gomes A M, Souza Carmo T, Silva Carvalho L, et al. (2018). Comparison of yeasts as hosts for recombinant protein production[J]. Microorganisms. 6(2): 38.
Figure 1. Main steps of protein production in yeasts.
