Escherichia coli (E. coli) has the advantages of clear genetic traits, rapid reproduction, economical culture, and high expression levels. Therefore, the E. coli expression system is the most widely used system in the development of gene expression technology. It is reported that the E. coli expression system is used to solublely express HPV proteins, and HPV virus-like particles are obtained through purification and recombination processes, which is helpful for the development of in vitro diagnostics (IVD) reagents and antibodies. Creative BioMart supports IVD development and produces large quantities of high-purity target proteins to meet customer needs.
Figure 1. A brief process for protein expression in E. coli.
Advantages of Our E. coli Protein Expression Service
Clear genetic background.
Fast reproduction, easy to cultivate and control, short cycle.
Low cost.
Strong anti-pollution ability and good stability.
High expression level, relatively simple separation and purification of expression products.
Commercial vectors and strains are complete and have a wide range of applications.
Easy conversion.
E. coli Protein Expression Service Items
Creative BioMart provides customers with services ranging from plasmid construction, transformation, strain screening, target protein expression and purification, inclusion body refolding, as well as property analysis to meet different needs.
Service Item
Service Process
Time (days)
Deliverables
Plasmid Construction
Codon optimization and gene synthesis
15-20
Expression plasmid
Sequencing report
Expression strain
Restriction enzyme digestion of PCR products
Vector connection
Transform into E. coli strains
Obtain the correct recombinant plasmid
Transformation and Strain Screening
Transfer recombinant plasmids into host cells and culture overnight
5
Related expression results
Select single colonies for small-scale induced expression
SDS-PAGE analysis of protein expression
Optimize expression conditions
Target Protein Expression and Purification
Large-scale expression of 1-10L based on optimized expression conditions
12-15
Purified protein
Protein purification
Inclusion Body Refolding
Protein denaturation and renaturation
2-4
Purified protein
Additional Services
Label removal services
2-5
Optimized high-quality and purity protein
Sterilization by filtration
Endotoxin removal
Freeze-drying
QC
Purity and concentration test
3-5
QC report
Provide QC report
Reasons to Choose Us
Flexible protein scale-up production.
Competitive price and fast delivery time.
Provide one-stop services from vector construction, protein expression, and protein purification to antibody preparation.
High-quality assurance, rationalized customized services to meet different needs.
High success rate and reliable results.
With a high-throughput screening platform, the optimal expression conditions can be quickly and efficiently obtained.
Creative BioMart focuses on IVD proteins, we can provide services such as small-scale and large-scale protein production for IVD and the development of recombinant proteins, antibodies, and bioanalytical tests, aiming to provide global pharmaceutical companies and life science research institutions with high-quality IVD protein service. If you are interested in our services, please contact us and we will provide you with detailed assistance.
Reference
Karyolaimos A, Ampah-Korsah H, Hillenaar T, et al. (2019). Enhancing recombinant protein yields in the E. coli periplasm by combining signal peptide and production rate screening[J]. Frontiers in microbiology. 10: 1511.
Figure 1. A brief process for protein expression in E. coli.
