Recombinant proteins are proteins obtained using recombinant DNA or recombinant RNA technology. Transient transfection is an efficient method used to obtain large amounts of a protein of interest in a short period, allowing researchers to identify drug candidates early by producing the protein in the early stages of drug development. Transient expression services can quickly and efficiently produce large amounts of recombinant proteins, which can be used as in vitro diagnostic (IVD) reagents, kits, or biosensors. At the same time, the kinases and toxin proteins produced by transient expression services are used to develop new drugs or treatment strategies, as well as conduct drug screening and research.
Figure 1. Rational design of transient gene expression process with lipoplexes for high-level therapeutic protein production in HEK293 cells. (Lee Y, et al., 2019)
Creative BioMart has rich experience in producing and purifying antibodies and is committed to providing comprehensive protein expression services for IVD to the research community.
Advantages of Our Transient Expression System
Low immunogenicity, no need for immunization and screening.
High batch-to-batch consistency and good repeatability.
High sensitivity and specificity.
Low endotoxin content.
Provide murine pathogen screening.
The cycle is short, and it can be produced and delivered in a few weeks.
Satisfying large-scale antibody preparation, animal-free in vitro production can be realized.
Creative BioMart uses CHO or HEK293 cells to produce high-level recombinant antibodies to meet the different needs of customers and facilitate the development of IVD.
Transient Expression Service Items
Proteins and antibodies are key raw materials for diagnostic reagents, which are used in immunology, tumors, allergies, etc. Creative BioMart has a one-stop protein service platform, providing customers with services from transient transfection, expression, and purification to delivery to meet different needs.
Service Item
Service Process
Time (days)
Deliverables
Plasmid Construction
Codon optimization and gene synthesis
10-15
Recombinant plasmid
Sequencing report
PCR amplification
Ligate to vector
Obtain the correct recombinant plasmid
Transfection
Transfection of various plasmids into HEK293 cells
12-17
Related identification results
Add enhancers to increase yield
Concentration of cell supernatant
Expression and Purification
Purify the supernatant with Protein A or G
10-15
Purified protein
Related identification results
Change buffer to PBS
Additional Services
Sterilization by filtration
2-5
Optimized high-quality protein
Endotoxin removal services
Mouse pathogen screening services
QC
Purity and concentration test
3-5
QC report
Provide QC report
Reasons to Choose Us
Extensive antibody expression experience facilitates the expression of various forms of full-length antibodies.
Provide one-stop services.
High detection success rate and stable antibody quality.
Deliverable results in a short period, saving time.
Provide an overall solution for personalized antibody discovery, with high expression of recombinant antibodies.
Provide endotoxin detection and removal services.
Creative BioMart focuses on IVD proteins, we can provide services such as small-scale and large-scale protein production for IVD and the development of recombinant proteins, antibodies, and bioanalytical tests, aiming to provide global pharmaceutical companies and life science research institutions with high-quality IVD protein service. If you are interested in our services, please contact us and we will provide you with detailed assistance.
Reference
Lee Y, Kim H, Kim E, et al. (2019). Rational design of transient gene expression process with lipoplexes for high-level therapeutic protein production in HEK293 cells[J]. Process Biochemistry. 85: 185-194.
Figure 1. Rational design of transient gene expression process with lipoplexes for high-level therapeutic protein production in HEK293 cells. (Lee Y, et al., 2019)
