Mycobacterium tuberculosis (MTB) is the most important and common pathogen causing tuberculosis (TB) in humans. It can invade various organ systems throughout the body, with lung infection being the most common infection. MTB is mainly spread through air, dust, and aerosol. A patient with a severe infection may suffer from hemoptysis as well as coughing and low-grade fever. Therefore, early detection and treatment of active TB is crucial. Bacillus Calmette-Guerin (BCG) is currently the only TB preventive vaccine approved for clinical use.
Figure 1. Life cycle of Mycobacterium tuberculosis. (Chandra P, et al., 2022)
Main Steps of IVD for Mycobacterium Tuberculosis
Tuberculin test (PPD test). Based on the principle of delayed skin hypersensitivity, tuberculin is injected into the skin. Those with redness, swelling, and induration exceeding 5 mm after two or three days are considered positive.
IFN-γ release assay. After injection of tuberculosis-related antigen, the interferon content in the serum was quantitatively detected. Increased interferon indicates possible Mycobacterium tuberculosis infection.
DNA Testing. It mainly includes PCR, Xpert MTB/RIF test, 16S rRNA highly variable region sequence determination, and restriction length polymorphism analysis.
Direct smear staining examination. Smear directly from expectoration or smear after collecting bacteria, and stain with acid-fast stain.
Isolation culture. The sample is inoculated into a solid medium and cultured under suitable conditions for some time. If cauliflower-like colonies appear, the infection is determined.
Creative BioMart provides high-quality recombinant Mycobacterium tuberculosis antigen protein used for IVD, including ELISA, lateral flow assays, western blots, and other immunoassays.
High sensitivity, high specificity, and high purity.
It is widely used and suitable for downstream immunological experiments.
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Outstanding success rate and fast development speed.
Our Outstanding Advantages
IVD proteins can be used to test for a variety of diseases and conditions, making them valuable tools for diagnosing and monitoring health.
Guarantee high performance, high reliability, and high consistency of protein quality, leading the industry.
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In addition, Creative BioMart also offers a series of viral proteins and protein-related services to provide customers with high-quality, low-cost active recombinant proteins to meet different needs and assist in preclinical drug development.
Creative BioMart offers a series of highly efficient and reliable IVD proteins, providing strong support for medical research and preclinical diagnosis. At the same time, we provide more flexible and personalized protein services that are used in IVD reagents, drug development, biotechnology research and production, and other fields. Please contact us for answers if you are interested in our products or services.
Reference
Chandra P, Grigsby S J, Philips J A. (2022). Immune evasion and provocation by Mycobacterium tuberculosis[J]. Nature Reviews Microbiology. 20(12): 750-766.
Figure 1. Life cycle of Mycobacterium tuberculosis. (Chandra P, et al., 2022)
