Recombinant Adenovirus Type 11 Particles
Specification
Related Products
| Cat#: |
ADE-005 |
| Product Name: |
Recombinant Adenovirus Type 11 Particles |
| Description: |
This product is a concentrated source of highly-purified human Adenovirus type 11 particles (wild-type) from a lysate of optimally-infected HEK293 cells. Following double CsCl gradient purification with DNase treatment and dialysis, our Ad11 preparation is of very high quality, with minimal lot-to-lot variation. |
| Gene: |
Adenovirus Type 11 Particles |
| Species: |
Adenovirus |
| Source: |
HEK293 |
| Synonyms: |
Adenovirus Type 11 Particles |
| Formulation: |
PBS,50 mM Hepes pH 7.8,10% Glycerol |
| Concentration: |
1.96x10^12 particles per ml (Picogreen assay) |
| Purity: |
Purified virus, double CsCl gradient purification with DNase treatment and dialysis. |
| Storage: |
Store frozen at less than -20 centigrade, preferably -80 centigrade. |
| Notes: |
This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations. |
| Freezing: |
Can be frozen, but avoid multiple freeze/thaw cycles. |
| Background: |
Adenoviruses are medium-sized (80–100 nm), non-enveloped viruses. They have an icosahedral nucleocapsid containing a linear, double-stranded DNA genome of approximately 36 kb (Nermut, 1984). The viral genome is grouped into different transcriptional units, designated early (E1, E2, E3, E4), intermediate, and late. The E1 gene is essential for activation of other viral genes and for viral replication. Deletion of the E1 gene results in viruses that are replication incompetent in normal cells. However, replication-competent viral particles can be produced from E1-deleted viral vectors by providing the E1 gene in trans. The E3 gene is nonessential for either viral replication or infection (Flint, 1999).
Adenovirus type 11 (Ad11) is associated with respiratory tract infections and conjunctivitis. It belongs to the B group of adenoviruses (along with Ad3), which use a different host cell receptor to Ad5 (Ad5 belongs to group C). Ad11 uses CD46 (and other receptors), whereas Ad5 binds the Coxsackie and adenovirus receptor (CAR). CAR expression is often down-regulated in human tumors, making Ad5-mediated gene transfer problematic. This has resulted in the development of chimeric Ad5/Ad11 vectors for improved oncolytic gene therapy. |
For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
