Recombinant MERS Coronavirus Spike Glycoprotein (S1), His-tagged
Specification
Related Products
| Cat#: |
HUM-312 |
| Product Name: |
Recombinant MERS Coronavirus Spike Glycoprotein (S1), His-tagged |
| Description: |
MERS Coronavirus Spike Glycoprotein (S1), His-Tag is a recombinant protein, cloned and expressed in E. coli. |
| Gene: |
S1 |
| Species: |
MERS Coronavirus |
| Source: |
E. coli |
| Synonyms: |
MERS Coronavirus Spike Glycoprotein (S1) |
| Purity: |
Purity >95% as determined by 10% PAGE (Coomassie staining). |
| Notes: |
This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations. |
| Applications: |
For use in ELISA and other immunoassays. |
| Tags: |
C-terminal 6xHis |
| Background: |
Middle East respiratory syndrome (MERS), also known as camel flu, is a viral respiratory infection caused by the MERS-coronavirus (MERS-CoV). MERS-CoV is a betacoronavirus derived from bats. Since April 2012, cases of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) have been identified in middle east and other countries, causing severe illnesses with ~35% mortality. Spread between humans typically requires close contact with an infected person. Camels have been shown to have antibodies to MERS-CoV but the exact source of infection in camels was not identified. Camels are also believed to be involved in transmission to humans. MERS-CoV genomes are phylogenetically classified into two clades, Clade A and B. Early cases of MERS were of Clade A clusters (EMC/2012 and Jordan-N3/2012), whilst more recent cases have been Clade B (Chu et al., 2014).
Three MERS-CoV proteins are expressed on the envelope of the virus: the surface spike protein (S), the membrane glycoprotein (M), and the envelope protein (E). The S protein is responsible for viral entry via attachment to and fusion with the host cell membrane. Spike protein has two domains, S1 and S2; the S1 domain is responsible for cellular tropism and interaction with target cell, whilst the S2 domain is responsible for membrane fusion. MERS-CoV has pandemic potential but there is currently no specific vaccine or treatment for the disease. However, the C terminus of S1 contains a receptor binding domain with potential for vaccine development and use in diagnostics (Xu et al., 2019). To this end, a peptide from amino acids 56 to 295 of spike protein S1 was expressed and purified from E. coli, with a 6 x His tag is attached to its C-terminus. |
For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
