Recombinant Measles Virus Nucleoprotein
Specification
Related Products
| Cat#: |
MEA-497 |
| Product Name: |
Recombinant Measles Virus Nucleoprotein |
| Description: |
Measles virus (Rubeola) recombinant nucleoprotein antigen is manufactured in mammalian HEK293 cells for use in diagnostics research and vaccine development. |
| Gene: |
NP |
| Species: |
Measles Virus |
| Source: |
HEK293 |
| Synonyms: |
Measles Virus Nucleoprotein |
| Formulation: |
Dulbecco's phosphate buffered saline (DPBS) pH 7.4. Contains traces of SDS. |
| Notes: |
This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations. |
| Background: |
Measles virus (MV) is the type species of the Morbillivirus genus, which is one of the seven genera of the family Paramyxoviridae. It is a highly contagious, re-emerging, major human pathogen. It is a globally widespread disease that affects children but can also cause disease in unvaccinated adults. Humans are the only known host of measles virus and infection is spread from person-to-person via respiratory aerosol droplets, nasal secretions or through direct contact with infected individuals.
MVs are enveloped and contain single-stranded, negative-sense RNA genomes of 9-19 kbp. The viral RNA is bound by thousands of copies of the viral nucleoprotein, forming the helical nucleocapsid (NC). The MV single-stranded RNA genome is enclosed by nucleocapsid proteins into helical ribonucleoprotein (RNP) complexes comprising of nucleo- (N), phospho- (P), and large (L) proteins. Encapsidated RNAs act as templates for transcription and replication by the viral RNA-dependent RNA-polymerases (RdRPs) complexes (Gutsche et al., 2015). Unencapsidated viral genomic RNA is transcriptionally inactive and not infectious (Duprex et al., 2002). N protein comprises two distinct parts: Folded Ncore and Ntail, an intrinsically disordered domain, containing a linear motif with α-helical propensity (residues 484–502) (Guseva et al., 2019).
MeV has an RNA-dependent RNA polymerase composed of two viral polypeptides, a large protein, L, and a phosphoprotein, P, for transcription and replication of their genome. The L protein confers the RNA polymerase activity on the complex while the P protein acts as a transcription factor. Replication requires a viral RNA-dependent RNA polymerase (RdRP) consisting of the large (L) polymerase protein complexed with the homo-tetrameric phosphoprotein (P) (Du Pont et al., 2019). |
For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
