Recombinant Hepatitis B Virus E Antigen (HBeAg)
Specification
Related Products
| Cat#: |
HEP-243 |
| Product Name: |
Recombinant Hepatitis B Virus E Antigen (HBeAg) |
| Description: |
Hepatitis B virus e antigen (HBeAg) expressed in E. coli. |
| Gene: |
HBeAg |
| Species: |
HBV |
| Source: |
E. coli |
| Synonyms: |
HBeAg |
| Formulation: |
25mM Tris-HCl, pH 8.0, 1.5 M urea, 50% glycerol. |
| Purity: |
95% by SDS-PAG |
| Notes: |
This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations. |
| Background: |
HBV is an enveloped DNA virus with a 3.2-kb covalently closed circular DNA, which replicates within the nucleus of infected hepatocytes (Seeger & Mason, 2015). Four genes are present on the virus genome; pre-core/core, polymerase (P), envelope (pre-S1/pre-S2/S) and X. The pre-core/core gene is responsible for expression of core protein as well as Hepatitis B e antigen (HBeAg), a serological marker of HBV infection correlating with high viral load.
The 5′ end of pre-S1, pre-S2 and S regions all have independent start codons which can each generate three co-terminal envelope proteins called large (L; pre-S1 pre-S2 S), middle (M; pre-S2 S), and small (S; with S domain alone) envelope proteins. The S protein is the most abundant envelope protein expressed and can be secreted independently of L and M envelope proteins. After secretion, mature HBeAg is deleted at residue 149 C-terminally and retains 10 pre-core residues N-terminally. Both L and S envelope proteins are needed for virion secretion, while M protein is dispensable (Bruss & Ganem, 1991; Ueda, et al., 1991).
Core particles with mature (double stranded DNA) genome can be enveloped and released as 42-nm virions, with the three envelope proteins inserted on the surface. Empty viral particles (22-nm) are also abundantly produced (Bruss & Ganem, 1991; Ganem & Prince, 2004; Zhang & Tang, 2017).
The exact function of this non-structural protein is unknown. HBeAg is dispensable for replication, as mutant viruses with defects in the pre-C region are both infectious and pathogenic (Mandell, et al., 2009). However, it is thought that HBe may be influential in suppressing the immune systems response to HBV infection by down-regulating Toll-like receptor 2 expression on hepatocytes and monocytes leading to a decrease in cytokine expression. The presence of HBeAg in patient serum can serve as a marker of active replication in chronic hepatitis. |
For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
