Recombinant Hepatitis B Virus Core Antigen (HBcAg)
Specification
Related Products
| Cat#: |
HEP-242 |
| Product Name: |
Recombinant Hepatitis B Virus Core Antigen (HBcAg) |
| Description: |
Recombinantly produced Hepatitis B Virus core antigen (HBcAg). This core protein fragment represents part of the infectious virions inner core particle, which encloses the viral genome. |
| Gene: |
HBcAg |
| Species: |
HBV |
| Source: |
E. coli |
| Synonyms: |
HBcAg |
| Formulation: |
10 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM EDTA, 50% glycerol |
| Concentration: |
1.0 mg/mL |
| Purity: |
>90% by SDS-PAGE |
| Storage: |
Short Term Storage: +2 centigrade to +8 centigrade
Long Term Storage: -80 centigrade |
| Notes: |
This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations. |
| Freezing: |
Can be frozen, but avoid multiple freeze/thaw cycles. |
| Background: |
HBV is an enveloped DNA virus with a 3.2-kb covalently closed circular DNA, which replicates within the nucleus of infected hepatocytes (Seeger & Mason, 2015). Four genes are present on the virus genome; pre-core/core, polymerase (P), envelope (pre-S1/pre-S2/S) and X. The pre-core/core gene is responsible for expression of core protein as well as hepatitis B e antigen (HBeAg), a serological marker of HBV infection correlating with high viral load.
The 5′ end of pre-S1, pre-S2 and S regions all have independent start codons which can each generate three co-terminal envelope proteins called large (L; pre-S1 pre-S2 S), middle (M; pre-S2 S), and small (S; with S domain alone) envelope proteins. The S protein is the most abundant envelope protein expressed and can be secreted independently of L and M envelope proteins. After secretion, mature HBeAg is deleted at residue 149 C-terminally and retains 10 pre-core residues N-terminally. Both L and S envelope proteins are needed for virion secretion, while M protein is dispensable (Bruss & Ganem, 1991; Ueda et al., 1991).
Core particles with mature (double stranded DNA) genome can be enveloped and released as 42-nm virions, with the three envelope proteins inserted on the surface. Empty viral particles (22-nm) are also abundantly produced (Bruss & Ganem, 1991; Ganem & Prince, 2004; Zhang & Tang, 2017). HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. It is a highly immunogenic subviral particle comprising 180 or 240 copies of the core protein and functions as both a T-cell-dependent and a T-cell-independent antigen. |
For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
