Recombinant Borrelia burgdorferi sensu stricto (B31) ErpN/OspE Protein, MBP-tagged
Specification
Related Products
| Cat#: |
BOR-031 |
| Product Name: |
Recombinant Borrelia burgdorferi sensu stricto (B31) ErpN/OspE Protein, MBP-tagged |
| Description: |
Recombinant Borrelia burgdorferi ErpN protein, fused to an MBP-tag and produced in E. coli (>90% purity). |
| Gene: |
ErpN/OspE |
| Species: |
Borrelia burgdorferi sensu stricto (B31) |
| Source: |
E. coli |
| Synonyms: |
Borrelia burgdorferi sensu stricto (B31) ErpN/OspE |
| Formulation: |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
| Concentration: |
1.0 mg/mL by UV absorbance at 280 nm |
| Stability: |
At +4 centigrade: Not determined.
At -80 centigrade: Not determined. |
| Purity: |
>90% by SDS-PAGE |
| Storage: |
Short Term Storage: +2 centigrade to +8 centigrade
Long Term Storage: -80 centigrade |
| Notes: |
This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations. |
| Tags: |
MBP |
| Freezing: |
Can be frozen, but avoid multiple freeze/thaw cycles. |
| Background: |
ErpN (OspE/F-Related Protein D), also known as Arp37, is encoded by the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. Strain B31 is the type strain (ATCC 35210) for this organism and was derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour (Johnson, et al., 1984). Several studies have demonstrated that infected humans and animals produce antibodies directed against Erp proteins within the first 2-4 weeks of infection, suggesting Erp synthesis occurs during the initial stages of vertebrate infection. Surface-exposed Erp proteins may then facilitate interactions with host tissues during the establishment of vertebrate infection (Fraser, et al., 1997).
Erp proteins from B. burgdorferi are believed to be lipoproteins, based on their predicted amino acid sequences. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host (Stevenson, et al., 1996). |
For research or industrial raw materials, not for personal medical use!
