Native Clostridium Difficile Toxoid A
Specification
Related Products
| Cat#: |
CLO-082 |
| Product Name: |
Native Clostridium Difficile Toxoid A |
| Description: |
Clostridium difficile Toxoid A is a highly purified, non-toxic preparation derived from C. difficile toxin A. It has been developed either for use as an immunogen to raise antibodies or for use in clinical diagnostic assays. Formaldehyde is use to convert the toxin into Toxoid A and quality control data illustrates that this treatment has no significant effect on the antigenicity of the resultant preparation. |
| Gene: |
Toxoid A |
| Species: |
Clostridium Difficile |
| Synonyms: |
Clostridium Difficile Toxoid A |
| Formulation: |
Contents when reconstituted in 250μl sterile distilled water will contain: Toxoid A at a concentration of 0.4 mg/mL in 0.05M Hepes, 0.15M NaCl and 5% sucrose |
| Stability: |
Lyophilised vial: 1 year after date of receipt.
Reconstituted liquid: 1 month |
| Purity: |
>95% pure by SDS-PAGE |
| Storage: |
Lyophilised vial: +4 centigrade.
Reconstituted liquid: +4 centigrade. |
| Notes: |
This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations. |
| Shipping: |
Ambient |
| Freezing: |
Reconstituted liquid can be frozen at -80 centigrade to extend stability to 1 year; however every freeze/thaw cycle will cause increased aggregation. Avoid multiple freeze/thaw cycles and multiple temperature fluctuations. |
| Inactivation: |
Inactivated using formaldehyde. No cell rounding in the sensitive verocytotoxicity assay at 2 μg/mL compared to active toxin lethal dose of 0.2 ng/mL. |
| Sequence Strain: |
VPI10463 |
| Background: |
Clostridium difficile (C. difficile) is a Gram-positive spore-forming anaerobic bacterium, first described in the mid-1930s. Recent studies have shown that C. difficile is predominantly associated with cases of infectious diarrhoea in patients after treatment with antibiotics (antibiotic-associated diarrhoea, AAD), or have imbalanced commensal gastrointestinal flora. C. difficile infection can cause severe disease and death in a significant number of cases and is recognised as a leading cause of severe gastrointestinal disease and AAD in hospitalised patients (Voth, DE). The severity of the disease in each case is determined by various factors, including the virulence of the C. difficile strain, the condition of the patient's normal gut flora and the individual's immune response to intestinal damage.
Toxins A and B have been identified as major C. difficile virulence factors, which are encoded by the tcdA and tcdB genes respectively. Both toxin A and toxin B have pro-inflammatory and cytotoxic activity, which disrupt the intestinal epithelium leading to extensive damage and cell death in the large intestine (Carter, GP).
In recent years, new hypervirulent strains of C. difficile, including ribotype 027 and 078, have emerged causing new epidemics of C. difficile in the developed world, and are a cause for significant concern within the global health care community (Ghose, C).
The product is presented in a choice of pack sizes and is lyophilised for ease of use. |
For research or industrial raw materials, not for personal medical use!
