Human Red Blood Cells, 10% Washed Pooled Cells


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Catalog:  RBD-077H
Source:  Red Blood Cells
Species:  Human
Product Overview:  Human Red Blood Cells 10% Washed Pooled Cells
Description:  Human whole blood is washed to remove the platelet rich plasma, buffy coat layer, and leukocytes (WBC). Red blood cells are supplied as a 10 percent suspension in phosphate buffered saline (PBS). Human red blood cells are useful for the titration of complement, adsorption procedures, testing for agglutinins/HA assays, and for the preparation of stroma as particulate reagents. Human red blood cells are perishable and are collected and processed upon receipt of your order.
Form:  0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Applications:  Suggested Applications: Biochemical Assay
Applications Notes: Human whole blood cells are used for complement titration, adsorption procedures, HA assays and for the preparation of stroma as particulate reagents.
Storage:  Store human washed pooled red blood cells at 4 centigrade prior to opening. Be advised that blood is a perishable product and exact shelf may depend on application.
Shipping:  Wet Ice
Synonyms:  Human Washed Pooled Cells; Human WPCs; Human Red Blood Cells; Human RBCs; erythrocytes
Figure 1:  Red Blood Cells-077H, 1.jpg
Figure Note 1:  Mammalian cell toxicity assays.
(A) HMPC hemolysis assay. Red blood cells were diluted to 2% with PBS and then incubated with HMPC over the concentration range 0.125-64 μg/mL.
(B) Cytotoxicity of HMPC towards HKC-8 cells. HKC-8 cells were treated with increasing concentrations of HMPC for 24 hours before assessing viability using a WST-1 assay.
(C) Cytotoxicity of HMPC towards HepG2 cells. HepG2 cells were treated with increasing concentrations of HMPC for 24 hours before assessing viability using a WST-1 assay.
All experiments were repeated twice in triplicate and data show the mean +/- standard deviation.
Figure Title 1:  Agglutination
Figure 2:  Red Blood Cells-077H, 2.jpg
Figure Title 2:  Agglutination
Physical State:  Liquid
Sex:  Mixed
Sterility:  Non-sterile
Preservative:  None
Stabilizer:  None
Expiration:  This product MAY be stable for up to two (2) weeks if properly stored and handled.
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For research or industrial raw materials, not for personal medical use!

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