Native Sheep Gamma Globulin Fraction


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Catalog:  GGF-303S
Species:  Sheep
Product Overview:  Sheep Gamma Globulin Fraction
Description:  Sheep gamma globulin blocking reagent is an ideal blocker for western blotting, ELISA, Immunohistochemistry and other detection assays. Gamma globulins are a class of globulins, identified by their position after serum protein electrophoresis. The most significant gamma globulins are immunoglobulins ("Igs"), more commonly known as antibodies, although some Igs are not gamma globulins, and some gamma globulins are not Igs. Globulin is one of the three main types of serum proteins, the others being albumin and fibrinogen. Some globulins are produced in the liver, while others are made by the immune system.
Form:  0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:  This product was prepared from normal Sheep serum by a multi-step process which includes delipidation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in precipitin arcs against anti-Sheep Serum corresponding to gamma globulins.
Applications:  Tested Applications: SDS-PAGE
Applications Notes: Sheep Gamma Globulin Fraction has been tested by SDS-PAGE and can be utilized in Western Blotting and ELISA experiments.
Storage:  Store vial at 4 centigrade prior to restoration. For extended storage aliquot contents and freeze at -20 centigrade or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 centigrade as an undiluted liquid. Dilute only prior to immediate use.
Concentration:  77.1 mg/mL by UV absorbance at 280 nm
Shipping:  Ambient
Reconstitution:  Restore with deionized water (or equivalent)
Synonyms:  Plasma Gamma Globulin; Serum Gamma Globulin; Globulin Fractions; Gammaglobulin; sheep gamma fraction
Figure 1:  Gamma Globulin Fraction-303S, 1.jpg
Figure Note 1:  SDS-PAGE results of Sheep Gamma Globulin.
Lane 1: reduced Sheep Gamma Globulin.
Lane 2: Opal PreStained Molecular Weight Ladder.
Lane 3: non reduced Sheep Gamma Globulin.
Load: 1.0 μg.
4-20% SDS Gel, Coomassie Blue Stained.
Figure Title 1:  SDS-PAGE
Physical State:  Lyophilized
Preservative:  0.01% (w/v) Sodium Azide
Stabilizer:  None
Expiration:  Expiration date is one (1) year from date of receipt.
Immunogen:  Not applicable
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For research or industrial raw materials, not for personal medical use!

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