Native Mouse Gamma Globulin Fraction


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Catalog:  GGF-104M
Species:  Mouse
Product Overview:  Mouse Gamma Globulin Fraction
Description:  Gamma globulins are a class of globulins, identified by their position after serum protein electrophoresis. The most significant gamma globulins are immunoglobulins ("Igs"), more commonly known as antibodies, although some Igs are not gamma globulins, and some gamma globulins are not Igs. Globulin is one of the three main types of serum proteins, the others being albumin and fibrinogen. Some globulins are produced in the liver, while others are made by the immune system.
Form:  0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:  Mouse Gamma Globulin was prepared from normal Mouse serum by a multi-step process which includes delipidation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in precipitin arcs against anti-Mouse Serum corresponding to gamma globulins.
Applications:  Tested Applications: SDS-PAGE
Suggested Applications: ELISA
Applications Notes: Mouse gamma globulin blocking reagent has been tested by SDS-PAGE and is an ideal blocker for western blotting, ELISA, Immunohistochemistry and other detection assays.
Storage:  Store gamma globulin at 4 centigrade prior to restoration. For extended storage aliquot contents and freeze at -20 centigrade or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 centigrade as an undiluted liquid. Dilute only prior to immediate use.
Concentration:  20.0 mg/mL by UV absorbance at 280 nm
Shipping:  Ambient
Reconstitution:  Restore with deionized water (or equivalent)
Synonyms:  Mouse Gamma-globulin; Plasma Gamma Globulin; Serum Gamma Globulin; Globulin Fractions; Gammaglobulin; mouse gamma fraction
Figure 1:  Gamma Globulin Fraction-104M, 1.jpg
Figure Note 1:  SDS results of Mouse Gamma Globulin Fraction.
Lane 1: Ms Gamma Globulin [2μg reduced].
Lane 2: Prestained MW marker.
Lane 3: Ms Gamma Globulin [2μg non-reduced].
Coomassie stained.
Predicted/Observed size: Reduced: 55 and 28 kDa, Non Reduced: ~160 kDa.
Figure Title 1:  SDS-PAGE
Figure 2:  Gamma Globulin Fraction-104M, 2.jpg
Figure Title 2:  ELISA
Physical State:  Lyophilized
Preservative:  0.01% (w/v) Sodium Azide
Stabilizer:  None
Expiration:  Expiration date is one (1) year from date of receipt.
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For research or industrial raw materials, not for personal medical use!

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