Native Goat Gamma Globulin Fraction


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Catalog:  GGF-038G
Species:  Goat
Product Overview:  Goat Gamma Globulin Fraction
Description:  Goat gamma globulin blocking reagent is an ideal blocker for western blotting, ELISA, Immunohistochemistry and other detection assays. Gamma globulins are a class of globulins, identified by their position after serum protein electrophoresis. The most significant gamma globulins are immunoglobulins ("Igs"), more commonly known as antibodies, although some Igs are not gamma globulins, and some gamma globulins are not Igs. Globulin is one of the three main types of serum proteins, the others being albumin and fibrinogen.
Form:  0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:  This product was prepared from normal serum by a multi-step process which includes delipidation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in precipitin arcs against anti-Goat Serum corresponding to gamma globulins.
Applications:  Tested Applications: ELISA, SDS-PAGE
Applications Notes: Goat Gamma Globulin Fraction has been tested by ELISA and SDS-PAGE and can be utilized in Western Blotting, ELISA experiments, and other detection assays.
Storage:  Store vial at 4 centigrade prior to restoration. For extended storage aliquot contents and freeze at -20 centigrade or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 centigrade as an undiluted liquid. Dilute only prior to immediate use.
Concentration:  40 mg/mL by UV absorbance at 280 nm
Shipping:  Ambient
Reconstitution:  Restore with deionized water (or equivalent)
Synonyms:  Plasma Gamma Globulin; Serum Gamma Globulin; Globulin Fractions; Gammaglobulin; goat gamma fraction
Figure 1:  Gamma Globulin Fraction-038G, 1.jpg
Figure Note 1:  SDS-PAGE results of Goat Gamma Globulin.
Lane 1: reduced Goat Gamma Globulin.
Lane 2: Molecular Weight Ladder.
Lane 3: non-reduced Goat Gamma Globulin.
Load: 1.0 μg. 4-20% SDS Gel, Coomassie Blue Stained.
Expect: reduced at 55, 25 kDa; non reduced 150-170 kDa, migrates higher.
Figure Title 1:  SDS-PAGE
Physical State:  Lyophilized
Preservative:  None
Stabilizer:  None
Expiration:  Expiration date is one (1) year from date of receipt.
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For research or industrial raw materials, not for personal medical use!

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